Review



readyprep sequential extraction kit  (Bio-Rad)


Bioz Verified Symbol Bio-Rad is a verified supplier
Bioz Manufacturer Symbol Bio-Rad manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Bio-Rad readyprep sequential extraction kit
    Readyprep Sequential Extraction Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/readyprep+sequential+extraction+kit/ReadyPrep+Sequential+Extraction+Kit/us12274741-290-26-30
    Average 93 stars, based on 114 article reviews
    readyprep sequential extraction kit - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Isolation:

    Article Title: Orchestrated control of filaggrin–actin scaffolds underpins cornification
    Article Snippet: Data acquisition was carried out on the Zeiss 780, Zeiss LSM 710 inverted confocal microscope (Zeiss, Jena, Germany), Nikon Eclipse E600 (Nikon, Tokyo, Japan), or Leica SP8 (Leica, Wetzlar, Germany) inverted confocal microscope by recording 2D images in different axial (3D) planes. .. Isolated epidermal sheets were incubated in 8 M urea buffer (ReadyPrep Sequential Extraction kit, Reagent 2 plus reducing reagent; Bio-Rad, Hercules, CA, USA) and sonicated in a water bath for 30 min. Lysates were spun at 4 °C (13,000 rpm, 15 min) and run on 7% TA gels (Life Technologies/ThermoFisher Scientific, Waltham, MA, USA) in Xcell SureLock Mini-Cell Electrophoresis System (Life Technologies/ThermoFisher Scientific, Waltham, MA, USA). .. Proteins were transferred onto PVDF membranes (iBlot Dry Blot system stacks and iBlot transfer device; Life Technologies/ThermoFisher Scientific, Waltham, MA, USA).

    Incubation:

    Article Title: Orchestrated control of filaggrin–actin scaffolds underpins cornification
    Article Snippet: Data acquisition was carried out on the Zeiss 780, Zeiss LSM 710 inverted confocal microscope (Zeiss, Jena, Germany), Nikon Eclipse E600 (Nikon, Tokyo, Japan), or Leica SP8 (Leica, Wetzlar, Germany) inverted confocal microscope by recording 2D images in different axial (3D) planes. .. Isolated epidermal sheets were incubated in 8 M urea buffer (ReadyPrep Sequential Extraction kit, Reagent 2 plus reducing reagent; Bio-Rad, Hercules, CA, USA) and sonicated in a water bath for 30 min. Lysates were spun at 4 °C (13,000 rpm, 15 min) and run on 7% TA gels (Life Technologies/ThermoFisher Scientific, Waltham, MA, USA) in Xcell SureLock Mini-Cell Electrophoresis System (Life Technologies/ThermoFisher Scientific, Waltham, MA, USA). .. Proteins were transferred onto PVDF membranes (iBlot Dry Blot system stacks and iBlot transfer device; Life Technologies/ThermoFisher Scientific, Waltham, MA, USA).

    Extraction:

    Article Title: Orchestrated control of filaggrin–actin scaffolds underpins cornification
    Article Snippet: Data acquisition was carried out on the Zeiss 780, Zeiss LSM 710 inverted confocal microscope (Zeiss, Jena, Germany), Nikon Eclipse E600 (Nikon, Tokyo, Japan), or Leica SP8 (Leica, Wetzlar, Germany) inverted confocal microscope by recording 2D images in different axial (3D) planes. .. Isolated epidermal sheets were incubated in 8 M urea buffer (ReadyPrep Sequential Extraction kit, Reagent 2 plus reducing reagent; Bio-Rad, Hercules, CA, USA) and sonicated in a water bath for 30 min. Lysates were spun at 4 °C (13,000 rpm, 15 min) and run on 7% TA gels (Life Technologies/ThermoFisher Scientific, Waltham, MA, USA) in Xcell SureLock Mini-Cell Electrophoresis System (Life Technologies/ThermoFisher Scientific, Waltham, MA, USA). .. Proteins were transferred onto PVDF membranes (iBlot Dry Blot system stacks and iBlot transfer device; Life Technologies/ThermoFisher Scientific, Waltham, MA, USA).

    Article Title: Horn fly protein as active antigen in anti-horn fly vaccine for protection of bovines against horn fly infestations
    Article Snippet: .. The adult flies used in the study were from the reference laboratory strain that has been kept in colony feeding upon citrated bovine blood at the USDA-ARS Knipling-Bushland U.S. Livestock Research Laboratory (KBUSLIRL) for over 50 years (Lohmeyer & Kammlah, Southwest Entomol., (2006) 31:83-5) Aqueous- and urea-extractible proteins were extracted using the ReadyPrep Sequential Extraction Kit (Bio-Rad) following the manufacturer's recommendation with a few modifications. ..

    Article Title: SNF2H interacts with XRCC1 and is involved in repair of H2O2-induced DNA damage.
    Article Snippet: The protein XRCC1 has no inherent enzymatic activity, and is believed to function in base excision repair as a dedicated scaffold component that coordinates other DNA repair factors.. Repair foci clearly represent the recruitment and accumulation of DNA repair factors at sites of damage; however, uncertainties remain regarding their organization in the context of nuclear architecture and their biological significance.. Here we identified the chromatin remodeling factor SNF2H/SMARCA5 as a novel binding partner of XRCC1, with their interaction dependent on the casein kinase 2-mediated constitutive phosphorylation of XRCC1.

    Article Title: Curcumin prevents proteins expression changes of oxidative phosphorylation, cellular stress response, and lipid metabolism proteins in liver of mice fed a high-fructose diet.
    Article Snippet: Increased fructose consumption has been associated with the development of metabolic diseases due to the modification in protein expression, altering metabolic and signaling pathways.. Curcumin is a natural compound with a regulatory effect on genes and metabolic pathways.. To identify the fructose-induced protein expression changes and the effect of curcumin on the change of protein expression in the liver of mice fed a standard diet and a high fructose diet, to elucidate the global role of curcumin.

    Article Title: Cattle fever tick-infestation vaccines and uses thereof
    Article Snippet: .. To determine if the selected tick proteins for evaluation as vaccine candidates could be detected in tick tissues, aqueous- and urea-extractible proteins were extracted using the ReadyPrep Sequential Extraction Kit (Bio-Rad) following the manufacturer's recommendation with a few modifications. ..

    Article Title: Comparison of fractionation proteomics for local SWATH library building
    Article Snippet: Oct4‐eGFP levels were monitored using 2 × 10 5 cells with an FC500 instrument (Beckman Coulter) and data was analyzed with FlowJo Analysis software. .. The rest of the cells was used to prepare cell lysates using R1‐buffer (Readyprep Sequential Extraction Kit, Bio‐Rad) containing 40 mM Tris pH 8.0, 1/100 Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fischer, cat. number 78 441) and 1/1000 Benzonase Nuclease (Sigma‐Aldrich, cat. number E1014‐25KU) at 1–10 × 10 6 cells/mL. ..

    Article Title: Identification of anti-horn fly vaccine antigen candidates using a reverse vaccinology approach
    Article Snippet: The adult flies used in the study were from the reference laboratory strain that has been kept in colony feeding upon citrated bovine blood at the United States Department of Agriculture (USDA)–Agricultural Research Services (ARS) Knipling-Bushland US Livestock Insects Research Laboratory (KBUSLIRL) for over 50 years [ ]. .. Aqueous- and urea-extractible proteins were extracted using the ReadyPrep Sequential Extraction Kit (Bio-Rad) following the manufacturer’s recommendation, with a few modifications. ..

    Article Title: Cattle fever tick-infestation vaccines and uses thereof
    Article Snippet: .. To determine if the selected tick proteins for evaluation as vaccine candidates could be detected in tick tissues, aqueous- and urea-extractible proteins were extracted using the ReadyPrep Sequential Extraction Kit (Bio-Rad) following the manufacturer's recommendation with a few modifications. ..

    Sonication:

    Article Title: Orchestrated control of filaggrin–actin scaffolds underpins cornification
    Article Snippet: Data acquisition was carried out on the Zeiss 780, Zeiss LSM 710 inverted confocal microscope (Zeiss, Jena, Germany), Nikon Eclipse E600 (Nikon, Tokyo, Japan), or Leica SP8 (Leica, Wetzlar, Germany) inverted confocal microscope by recording 2D images in different axial (3D) planes. .. Isolated epidermal sheets were incubated in 8 M urea buffer (ReadyPrep Sequential Extraction kit, Reagent 2 plus reducing reagent; Bio-Rad, Hercules, CA, USA) and sonicated in a water bath for 30 min. Lysates were spun at 4 °C (13,000 rpm, 15 min) and run on 7% TA gels (Life Technologies/ThermoFisher Scientific, Waltham, MA, USA) in Xcell SureLock Mini-Cell Electrophoresis System (Life Technologies/ThermoFisher Scientific, Waltham, MA, USA). .. Proteins were transferred onto PVDF membranes (iBlot Dry Blot system stacks and iBlot transfer device; Life Technologies/ThermoFisher Scientific, Waltham, MA, USA).

    Electrophoresis:

    Article Title: Orchestrated control of filaggrin–actin scaffolds underpins cornification
    Article Snippet: Data acquisition was carried out on the Zeiss 780, Zeiss LSM 710 inverted confocal microscope (Zeiss, Jena, Germany), Nikon Eclipse E600 (Nikon, Tokyo, Japan), or Leica SP8 (Leica, Wetzlar, Germany) inverted confocal microscope by recording 2D images in different axial (3D) planes. .. Isolated epidermal sheets were incubated in 8 M urea buffer (ReadyPrep Sequential Extraction kit, Reagent 2 plus reducing reagent; Bio-Rad, Hercules, CA, USA) and sonicated in a water bath for 30 min. Lysates were spun at 4 °C (13,000 rpm, 15 min) and run on 7% TA gels (Life Technologies/ThermoFisher Scientific, Waltham, MA, USA) in Xcell SureLock Mini-Cell Electrophoresis System (Life Technologies/ThermoFisher Scientific, Waltham, MA, USA). .. Proteins were transferred onto PVDF membranes (iBlot Dry Blot system stacks and iBlot transfer device; Life Technologies/ThermoFisher Scientific, Waltham, MA, USA).

    Modification:

    Article Title: Curcumin prevents proteins expression changes of oxidative phosphorylation, cellular stress response, and lipid metabolism proteins in liver of mice fed a high-fructose diet.
    Article Snippet: Increased fructose consumption has been associated with the development of metabolic diseases due to the modification in protein expression, altering metabolic and signaling pathways.. Curcumin is a natural compound with a regulatory effect on genes and metabolic pathways.. To identify the fructose-induced protein expression changes and the effect of curcumin on the change of protein expression in the liver of mice fed a standard diet and a high fructose diet, to elucidate the global role of curcumin.



    Similar Products

    93
    Bio-Rad readyprep sequential extraction kit
    Readyprep Sequential Extraction Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/readyprep+sequential+extraction+kit/ReadyPrep+Sequential+Extraction+Kit/us12274741-290-26-30
    Average 93 stars, based on 1 article reviews
    readyprep sequential extraction kit - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    93
    Bio-Rad urea buffer
    Urea Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/readyprep+sequential+extraction+kit/ReadyPrep+Sequential+Extraction+Kit+Reagent+2/pmc11257952-255-18-20
    Average 93 stars, based on 1 article reviews
    urea buffer - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    93
    Bio-Rad pellet
    Pellet, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/readyprep+sequential+extraction+kit/ReadyPrep+Sequential+Extraction+Kit/us11951159-296-1-32
    Average 93 stars, based on 1 article reviews
    pellet - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    93
    Bio-Rad aqueous soluble proteins
    Aqueous Soluble Proteins, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/readyprep+sequential+extraction+kit/ReadyPrep+Sequential+Extraction+Kit/us11951159-290-0-16
    Average 93 stars, based on 1 article reviews
    aqueous soluble proteins - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    93
    Bio-Rad sequential extraction kit
    Sequential Extraction Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/readyprep+sequential+extraction+kit/ReadyPrep+Sequential+Extraction+Kit/pm37073328-170-5-11
    Average 93 stars, based on 1 article reviews
    sequential extraction kit - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    Image Search Results